69
c. Cell stretch: After the cell is captured, the axial force acting on the cell is increased
by increasing the laser power, causing the cell to axially stretch.
d. Cell rotation: After the cells have completed the tensile deformation and restored to
the original state. Proper electrical signals are applied to the four thick electrodes to
cause the cell to rotate horizontally. e electrical properties of the cell are measured
by tting analysis of the rotation spectrum.
e. Cell recovery: After the rotation is completed, the height of the cell solution con-
tainer is lifted to ush the cell away from the working area to recover.
3.6 SINGLECELL MANIPULATION AND MULTIPARAMETER
ANALYSIS EXPERIMENTS
e cell sample preparation in the experiment was the same as that in Section 2.6.1. Five types
of cells are selected in this experiment, HeLa cells, A549 cells, HepaRG cells, MCF7 cells, and
MCF10A cells.
3.6.1 EXPERIMENTAL DEMONSTRATION OF FILTER MIRROR
In experiment, a 980-nm lter was placed in front of the CCD camera to eliminate the inuence
of CCD overexposure. Figure 3.12 shows the eect of the lter on dual-beam laser capture of a
MCF10A cell. If there was no lter, the laser scattered on the cell, and the image on the CCD is
aring, and the cell contour was hardly observable (Figure 3.12(a)). Figure 3.12(b) shows the image
with the lter, eectively eliminating the eects of overexposure caused by scattered light.
(a) (b)
Figure 3.12: Dual beam capture of one MCF10A cells: (a) without lter and (b) with lter.
3.6 SINGLECELL MANIPULATION AND MULTIPARAMETER ANALYSIS
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