UNIT 03

INVERTEBRATE INSPECTION

IF YOU GO BY NUMBERS, INVERTEBRATES RULE THE WORLD. These spineless marvels make up well over 90 percent of the animal species that have been identified on Earth and include everything from insects and arachnids to snails, worms, and single-celled creatures called protozoa.

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Some are so small that you can only view them under a microscope, while others, like giant tube worms living near toxic, deep sea ocean vents, can grow to be over seven feet (2.1 m) long.

There are lots of experiments you can do to learn about your invertebrate neighbors. In addition to identifying them, you can observe their behavior and even bring them to the surface to study more closely. You’ll find them fascinating.

In this chapter, you’ll learn to make a bug house, sweep for arthropods, fish for planaria, and irritate earthworms.

LAB 10

BUG HOUSE

CREATE CONNECTED MICROENVIRONMENTS TO LEARN ABOUT PILL BUGS AND SOW BUGS.

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MATERIALS

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Image 10 to 20 sow bugs or pill bugs (see Protocol for finding and catching them)

Image Jar or other container for bringing the bugs home

Image Rectangular plastic box and piece of cardboard, or 2 half-gallon (1.9 L) cardboard milk cartons

Image Scissors and/or utility knife

Image Duct tape or masking tape

Image Paper towels or dirt

Image Black or brown construction paper

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SAFTEY TIPS & HINTS

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— Adult supervision is required when using a utility knife to cut holes.

— Do not do this experiment with spiders or other insects that can bite or sting. If you live in an area with poisonous snakes, use caution moving rocks and logs while you look for bugs.

PROTOCOL

STEP 1: Collect sow bugs or pill bugs from under rocks and pieces of wood. These bugs are 1/4 inch (6 mm) to 1/2 inch (13 mm) long and have seven pairs of legs and a segmented body, like armor. Pill bugs are also called roly polys and can roll up into tiny balls. (Fig. 1, 2)

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Fig. 1: Collect sow bugs and pill bugs.

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Fig. 2: Look under rocks and pieces of wood.

STEP 2: Cut a snug-fitting piece of cardboard to divide your plastic container in half. Cut a small opening about 2 inches (5 cm) wide into the middle of the bottom of the cardboard for bugs to move through. Tape the cardboard container into place. (Fig. 3)

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Fig. 3: Make a bug house from a divided plastic container.

For milk cartons, cut the tops off, leaving 4 inches (10 cm) on the bottom. Cut identical holes in the side of each carton about 1/3 inch (1 cm) from the bottom. Tape the cartons together so the holes line up to form a single opening. (Fig. 4)

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Fig. 4: You can also make a bug house from milk cartons.

STEP 3: Add wet paper towels or dirt to one side of the habitat and dry towels or dirt to the other side so the bugs can crawl back and forth easily between the two sides.

STEP 4: Place an equal number of bugs on each side of the habitat and observe them for an hour or so. Every 15 minutes, record how many bugs are on the moist side and dry side of the environment. (Fig. 5)

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Fig. 5: Observe the bugs’ behavior.

STEP 5: Repeat the experiment making one side of the habitat dark by covering it with the construction paper, and leaving the other side open to the light. Add an equal number of bugs to each side and see which habitat they prefer.

STEP 6: Release the bugs back where you collected them.

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CREATIVE ENRICHMENT

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Try creating other microenvironments for the isopods. See what foods they prefer.

LAB 11

SWEEP NET ARTHROPOD INSPECTION

COLLECT AND IDENTIFY AMAZING ARTHROPODS USING A NET YOU CAN MAKE YOURSELF.

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MATERIALS

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Image Sweep net or materials to make one: pliers; two wire hangers; scissors; an old, light-colored pillowcase; duct tape; and long wooden broomstick or sturdy yardstick

Image Large white piece of fabric, such as an old sheet

Image Jars

Image Insect identification books (optional)

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SAFTEY TIPS & HINTS

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— Don’t pick up insects with your bare hands unless you know they don’t bite or sting.

— Ticks love tall grass. If there are ticks in your area, take precautions and do a tick check after your insect hunt.

PROTOCOL

STEP 1: If you don’t have a sweep net, make one by using pliers to straighten two wire hangers and twist them together. Form them into a loop, leaving about 3 inches (7.5 cm) straight on either end. Cut about one third off of the open end of a pillowcase and pull the mouth of the pillowcase over the wire loop, leaving the 3-inch (7.5 cm) extension bare. Tape it securely around the perimeter. To complete the net, tape the straight part of the hangers onto the end of a broomstick or yardstick. (Fig. 1)

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Fig. 1: Make a sweep net from wire hangers and a pillowcase.

STEP 2: Find an area with long grass and plants, such as a meadow or field. Sweep with your net the same way you’d sweep a floor, but flip the open side of the net back and forth to capture insects in the grass. (Fig. 2, 3)

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Fig. 2: Get ready to sweep.

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Fig. 3: Sweep the grass and plants to catch invertebrates.

STEP 3: Close your net by flipping the bottom over the top and carry it over to your fabric.

STEP 4: Carefully release the creatures you’ve collected onto the fabric to inspect them. If you want a closer look, use a leaf or stick to pick up an insect and put it inside a jar with a loose lid. (Fig. 4)

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Fig. 4: Observe your catch more closely in jars.

STEP 5: Count how many legs and body segments they have and look for antennae, wings, and unique color. Record your observations in a notebook.

STEP 6: If you want, you can use insect identification books or other means to identify what you’ve found. (Fig. 5)

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Fig. 5: Try to identify what you caught in your sweep net.

STEP 7: Keep a journal of the insects and arachnids you capture, the time of day, and where you found them.

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CREATIVE ENRICHMENT

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1. Sweep sections of the same area at different times of day (dawn, noon, dusk, and night) to see how the population changes.

2. Compare what you find in different habitats, for instance, prairie versus marsh.

LAB 12

FISHING FOR FLATWORMS

CATCH AND OBSERVE FASCINATING, FREE-LIVING PLANARIA.

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MATERIALS

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Image Knife that can cut meat

Image Raw meat such as steak or liver

Image String or fishing line with (optional) fish hook

Image Metal sinker or rock

Image Collection container such as a jar

Image Magnifying glass

Image Microscope with a petri dish or slides (optional)

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SAFTEY TIPS & HINTS

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— Never leave young children unattended near water.

— Always wash your hands after handling raw meat.

— If you don’t have any luck catching flatworms, look for them on the bottom of large, flat rocks sitting in clear, still water. Use a paintbrush to sweep them into your collection container.

PROTOCOL

STEP 1: Search for a spot, such as a pond, lake, or small stream, to catch flatworms called planaria. They prefer shade and still, clean water, often hiding out near docks, lily pads, large, flat stones, and debris. Flatworms are more active at night.

STEP 2: Cut meat into coin-size pieces and tie it to long pieces of string or use it to bait a fishing hook. Add a sinker near the meat or tie a rock on to the string. (Fig. 1)

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Fig. 1: Put meat on a string with a weight.

STEP 3: Drop your bait into the water and wait 5 or 10 minutes. Set out more bait in different spots and at different depths while you wait.

STEP 4: Fill a collection container with water from the fishing area.

STEP 5: When it’s time, pull the flatworm bait up slowly and lower it into the water in your container. (Fig. 2)

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Fig. 2: Pull the bait from the water and put it in a jar.

STEP 6: Use a magnifying glass to look for flatworms on the meat. They have a soft, unsegmented, flat body and an arrow-shaped head. If you don’t find any flatworms, lower the bait into the water and leave it for 3 to 4 hours before checking again. (Fig. 3)

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Fig. 3: Use a magnifying glass to look for flatworms.

STEP 7: Observe the planaria you catch with your magnifying glass and under a microscope, if you have one. Draw a planarium in your science notebook and record the behavior you observe. (Fig. 4)

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Fig. 4: Observe the planaria you catch.

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CREATIVE ENRICHMENT

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1. Study how planaria respond to light.

2. Try to keep your planaria alive by feeding them fish food and changing the water on occasion. They prefer cool temperatures, and chlorine will kill them, so use bottled water.

LAB 13

EARTHWORM ERUPTION

USE GROUND MUSTARD SEED DISSOLVED IN WATER TO MAKE EARTHWORMS WRIGGLE TO THE SURFACE WHERE YOU CAN CATCH THEM.

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MATERIALS

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Image1/3 cup (48 g) ground mustard

Image 1 gallon (about 4 L) water

Image Empty milk jug, bucket, or other large container

Image String, sticks, or stakes

Image Container for earthworms

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SAFTEY TIPS & HINTS

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— Don’t splash the mustard water in your eyes. It will sting!

— We’ve had the best luck catching lots of worms in grassy yards.

PROTOCOL

STEP 1: Add the ground mustard to the water in the jug, bucket, or large container and stir until dissolved. (Fig. 1)

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Fig. 1: Stir ground mustard into water.

STEP 2: Measure out a 1-square-foot (30.5 × 30.5 cm) sample plot with string and sticks or stakes.

STEP 3: Pour about half of the water-mustard mixture over the dirt in your grid. (Fig. 2)

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Fig. 2: Pour the water-mustard mix over the ground.

STEP 4: Wait for the worms to come up. When they’ve emerged completely, grab them and put them in a container. (Fig. 3)

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Fig. 3: Catch worms!

STEP 5: When worms stop emerging, add the rest of the mustard mixture to the plot and wait for a second batch of worms to appear from deeper in the soil.

STEP 6: Observe your earthworms. (Fig. 4)

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Fig. 4: Observe the earthworms.

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CREATIVE ENRICHMENT

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1. Measure out plots in different environments to see how many worms you catch.

2. Identify the worms you find and send them, or report your findings, to a citizen science project (see Resources).

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